Effect of different preparation procedures on the ultrastructure of gonococci.
نویسندگان
چکیده
Culture samples of Neisseria gonorrhoeae and Escherichia coli were studied by transmission and electron microscopy to evaluate the effect of different preparation procedures on the pilation of these organisms. Unfixed as well as fixed bacteria showed only few, long, filamentous appendages when investigated in ultrathin sections, negatively stained specimens, or critical-point dried preparations. Snap-frozen specimens of E. coli showed many short and thin pili after being shadowed with carbon and platinum whereas those of N. gonorrhoeae showed only some type-C-like pili. Thus, the number and morphological appearance of pili appear to be greatly influenced by the preparation techniques used for study by electronmicroscopy. Conclusions as to the type and the infectivity of a bacterial strain can, therefore, not be based on purely morphological criteria.
منابع مشابه
Effects of Fluvoxamine on sex Steroids and oocyte ultrastructure in three spot Gourami (Trichogaster trichopterus) after increasing dopamine level by Bromocriptine injection
Background: Effect of drugs on different receptors may cause effect and also unexpected side effects. For example, using Dopamine as an anti-parkinson drug block the upper GnRH axis and thatchr('39')s why some sexual disorders happen. In this study, the effect of serotonin as Fluvoxamine injection on lower levels of GnRH by blocking the upper GnRH axis with injection of Bromocriptine was inves...
متن کاملThe Effect of Chronic Spinal Cord Injury on Ultrastructure of Epididymal Sperm in Rat
Purpose: The effects of chronic SCI on ultrastructure of spermatozoa aspirated from epididymis of rats. Materials and Methods: 45 adults Wistar rats were divided into 3 groups of SCI, control and sham. Following laminectomy, SCI was induced with a 15g weight dropped from a distance of 10 cm, onto exposed dura matter at T10 level. Sham group underwent laminectomy of T10 only, while control was n...
متن کاملEffect of Helium-Neon Laser Irradiation on Motility and Ultrastructure of Fresh and Frozen-Thawed Human Sperm
Purpose: Semen freezing is an important technique in human infertility treatment in many different patients but it damages sperms. Low-power lasers are physical agents that have biostimulatory effects. In this study, low-power helium-neon laser's (16J/cm2, one time irradiation) effect on motility and ultrastructure of fresh and frozen-thawed human sperm were studied. Materials and Methods: Thi...
متن کاملThe Effect of Cardio Gel and Matrigel on the Ultrastructure of Cardiomyocytes Derived From Mouse Embryonic Stem Cells
Purpose: To investigate the effect of cardiogel and matrigel on the ultrastructure of embryonic stem cell-derived cardiomyocytes. ECM: Extracellular Matrix derived from cardiac fibroblasts (cardiogel), commercial extracellular matrix (matrigel) and control group (without ECM) were cultured for up to 21 days. Ultrastructural properties of cardiomyocytes were evaluated by transmitting electron mi...
متن کاملUltrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy
The leaf cuticular ultrastructure of some plant species has been examined by transmission electron microscopy (TEM) in only few studies. Attending to the different cuticle layers and inner structure, plant cuticles have been grouped into six general morphological types. With the aim of critically examining the effect of cuticle isolation and preparation for TEM analysis on cuticular ultrastruct...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The British journal of venereal diseases
دوره 55 2 شماره
صفحات -
تاریخ انتشار 1979